Journal: Clinical Cancer Research

Article Title: Vascular Endothelial Growth Factor Receptors VEGFR-2 and VEGFR-3 Are Localized Primarily to the Vasculature in Human Primary Solid Cancers

doi: 10.1158/1078-0432.ccr-09-2797

Figure Lengend Snippet: Fig. 1. Specificity of VEGFR-2 and VEGFR-3 antibodies. A, the specificity of antibodies raised to VEGFR-2 (55B11, sc-315, sc-504, and sc-6251) and VEGFR-3 (AF349) was determined by Western blot analysis of protein extracts from PAE and NIH-3T3 cells engineered to overexpress VEGFR-1, VEGFR-2, VEGFR-3, and CSF1R and cells expressing endogenous PDGFR-β (VSMC) and c-Kit (M-07e). B, 55B11 and AF349 were evaluated by immunocytochemical analysis of FFPE PAE cells engineered to overexpress VEGFRs.

Article Snippet: To do this, we determined the crossreactivity of human VEGFR antibodies using multiple www.aacrjournals.org Researc on Januarclincancerres.aacrjournals.org Downloaded from screening platforms and identified two antibodies, 55B11 (VEGFR-2; Cell Signaling Technology) and AF349 (VEGFR-3; R&D Systems), with credible specificity.

Techniques: Western Blot, Expressing

Journal: Experimental and Therapeutic Medicine

Article Title: Expression levels of VEGF-C and VEGFR-3 in renal cell carcinoma and their association with lymph node metastasis

doi: 10.3892/etm.2021.9986

Figure Lengend Snippet: Immunohistochemical staining of VEGFR-3 expression. VEGFR-3 immunohistochemical staining in (A) normal renal tissues and (B) RCC tissues. (C) Lymphatic endothelial cells in RCC tissues were VEGFR-3 + (indicated by arrows). Scale bars, 100 µm. (D) Correlation between VEGF-C and VEGFR-3 expression in RCC tissues. Statistical significance was determined by Spearman's correlation test. RCC, renal cell carcinoma; VEGF-C, vascular endothelial growth factor-C; VEGFR-3, VEGF receptor-3.

Article Snippet: For antigen retrieval, the tissue sections were incubated with 0.01 M sodium citrate (pH 6) in a microwave oven at 95˚C for 10 min, followed by blocking with 5% normal goat serum (cat. no. ZLI-9021; OriGene Technologies, Inc.) for 10 min at room temperature, the tissue sections were incubated with rabbit anti-human VEGF-C monoclonal antibody (cat. no. BA0548; 1:200; Wuhan Boster Biological Technology Co., Ltd.), rabbit anti-human VEGFR-3 monoclonal antibody (cat. no. A01276-3; 1:200; Wuhan Boster Biological Technology Co., Ltd.) and mouse anti-human D2-40 monoclonal antibody (cat. no. ZM-0465; undiluted; OriGene Technologies, Inc.) for 12 h at 4˚C.

Techniques: Immunohistochemical staining, Staining, Expressing